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Barry Selinsky: Membrane Protein Protocols : Expression, Purification, and Characterization

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Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.

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Shuzo Oshimi: Flowers of Evil: Vol. 3

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In the third volume of The Flowers of Evil, Takao must make a decision. He now has an opportunity to break free from the social shackles that Nakamura has placed on him, as he has a chance to be loved by someone he has affections for. This could also be an opportunity to be accepted by the masses in his small community. But with this choice comes fear and self-reflection. After having an chance trip to Nakamura's place, where he got to experience the world his only "friend" has grown up in, he realizes that for both teens, there is very little future to look forward to as they are raised in the boonies. Their lives are mapped out before them. For most adolescents these days, life will continue to be blase at best, miserable at worst. Can they change things together? Do they want to even bother?

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Alan Taylor: American Colonies: v. 1: The Settlement of North America to 1800

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AMERICAN COLONIES starts with the earliest years of human colonization of the American continent and environs with the Siberian migrations across the Bering Strait 15,000 years ago. It ends in around 1800 when the rough outline of the contemporary North America could be perceived. Dropping the usual Anglocentric description of North America's fate, Taylor brilliantly conveys the far more vivid and startling story of the competing interests--Spanish, French, English, Native, Russian--that over the centuries shaped and reshaped both the continent and its 'suburbs' in the Caribbean and the Pacific. It is one of the greatest of all human stories.

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K. Prakash: Paisley Designs

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Exotic and enduring, paisley patterns have decorated textiles and handicrafts for thousands of years. Boasting superb reproductions in vivid color and striking black-and-white, this treasury showcases 199 resplendent examples of traditional Indian and European paisley motifs.A spiritual affinity with the natural world isapparent throughout the collection, exhibited in lush designs inspired by trees, leaves, and flowers, as well as repeating geometric motifs. Brimming with a wealth of intricate details, this very special book is a unique and valuable resource for fashion and graphics designers, textile artists, needleworkers, and other craft enthusiasts, as well as art students and historians."

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A. Meredith Walters: Find You in the Dark

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Maggie Young had the market on normal. Normal friends, normal parents, normal grades...normal life. Until him. Clayton Reed was running from his past and an army of personal demons that threatened to take him down. He never thought he had a chance at happiness. Until her. Maggie thought their love could overcome anything. Clay thought she was all he needed to fix his messy life. That together, they could face the world. But the darkness is always waiting. Sometimes the greatest obstacle to true love is within yourself.

Since 1898, Model Engineer magazine (originally weekly, now fortnightly) has proved a remarkable source of knowledge for all manner of engineering practices. Its readership has always included many highly experienced engineers, some of considerable eminence, who have answered readers' questions and contributed valuable information in order that everyone the construction and use of machines should be able to extend his scope and skills. The answer to virtually any engineering problem can be found somewhere in its pages, it is said. Many of the magazine's most helpful nuggets of knowledge have appeared in brief notes or articles, often many years ago, and have never been republished. This book offers a selection of fascinating hints and Time Pieces for Cello: v. 1: Music Through the Ages download PDF tips culled from the magazine's wide time-scale (from the 1890s to the 1960s) but as relevant today as when they were first printed. They may save the reader time and possibly money; certainly they will interest and instruct anyone who works with metal.

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Time Pieces is an exciting new series of repertoire which explores the wealth of music written from the sixteenth century to the present day. The pieces are presented chronologically within each volume and all include the year in which they were written too so you can map your journey through time. Time Pieces for Cello is a three-volume anthology providing a graded selection of well-crafted and idiomatic arrangements, as well as some original pieces, for cello and piano. All of them have been carefully written to provide practice of the keys, note ranges and fingering patterns encountered in the early grades, and there are suggestions for position work and varied bowing patterns. Each volume balances well-known pieces with less familiar music, and the emphasis throughout is on tuneful repertoire perfectly suited to the cello with a simple supportive piano accompaniment. From Tallis to Tchaikovsky and from Haydn to Britten, Time Pieces for Cello allows the pupil to get a real feel for the different styles of music through tune and will prove an invaluable, varied source of further study and recital repertoire.

Since 1898, Model Engineer magazine (originally weekly, now fortnightly) has proved a remarkable source of knowledge for all manner of engineering practices. Its readership has always included many highly experienced engineers, some of considerable eminence, who have answered readers' questions and contributed valuable information in order that everyone the construction and use of machines should be able to extend his scope and skills. The answer to virtually any engineering problem can be found somewhere in its pages, it is said. Many of the magazine's most helpful nuggets of knowledge have appeared in brief notes or articles, often many years ago, and have never been republished. This book offers a selection of fascinating hints and Time Pieces for Cello: v. 1: Music Through the Ages download PDF tips culled from the magazine's wide time-scale (from the 1890s to the 1960s) but as relevant today as when they were first printed. They may save the reader time and possibly money; certainly they will interest and instruct anyone who works with metal.

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Stan Bray: Model Marine Steam

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This book provides all the information any ship modeller interested in powering a model boat using live steam will need. It offers both the basic theory covering the steam power plant and fully detailed drawings for the construction of simple and advanced steam engines, boilers and ancillary equipment. There has been a huge growth in interest in live steam-powered model boats in recent years but modellers have endured a dearth of practical construction drawings for suitable steam plants. Here, the author covers many types of engine from simple oscillating cylinder types to piston and poppet valve engines and the application of radio control to the management of the boiler and engine. The projects (which all include detailed scale plans) include simple single cylinder oscillating engines, multiple cylinder oscillating engines, single and multiple cylindered slide valve engines, piston and poppet valve engines, boiler construction and heat sources, control units, reversing systems, radio control valves, and the mounting of engine and boiler units into model boats.

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E T Barton,Robin E Davis: How to Start a Lucrative Virtual Bookkeeping Business: A Step-By-Step Guide to Working Less and Making More in the Bookkeeping Industry

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WARNING: This is not your typical bookkeeping book. You will not find a lot of "Industry Jargon" in this book, nor will you find your usual "do it this way because it's always been done this way" kind of advice. The authors of this book have a twisted sense of humor and it shows. (Their defense - staring at computer screens everyday has finally made them go batty.) This book was written only for the bookkeepers who are dying to try something new, easy and simple, and who don't want to do things "the same old way." If that's you, read on... *********************************** If you have dreams of starting your own lucrative virtual bookkeeping business, but have been putting it off because you're just not sure you can do it, or even get any clients, you're not alone. Every independent bookkeeper feels that way at one time or another. "How to Start a Lucrative Virtual Bookkeeping Business" was created to help get you past your own objections, create the perfect home office, and then go out and find the ideal virtual bookkeeping client - step-by-step. In this book, you will find: 10 Simple Steps to starting and running your virtual bookkeeping business. More than 30 ways to find new clients, including what-not-to-do. An additional 30 tips to help supplement your bookkeeping business. Scripts to turn your existing clients (or boss) into virtual bookkeeping clients. Advice on how to brand and market your new business on an extremely-tight budget. Multiple ways to get your clients to pay. How to recognize the warning signs of a Deadbeat Client. Advice from other bookkeeping business professionals on creating the virtual bookkeeping business you've always dreamed of. PLUS, there are links inside the book to a Bonus Page where you can download FREE "Printable" Word and PDF documents to help you implement the strategies easily into your own business. What are you waiting for? It's time to tell your boss you're making your own hours and they can take you or leave you as you are - a lucrative virtual bookkeeper business owner. *********************************** E.T. Barton and Robin Davis met back in 1976, shortly after Robin's "water broke." That's right, E.T. and Robin are mother and daughter and Robin was all of 5 years old at the time (at least that's the story Robin's sticking to!). Both mother and daughter got into bookkeeping shortly after high school (because it's in the genes), and through the years have encouraged each other to begin their own bookkeeping companies. Since E.T's passion lies in writing, and Robin's lies in bookkeeping, they've decided to team up and share their collective knowledge in a way that is simple and entertaining for the "Average Business Joe." Oh - and Bookkeepers will like it too."

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Henry A. Giroux: Neoliberalism's War on Higher Education

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"Neoliberalism s War on Higher Education" reveals how neoliberal policies, practices, and modes of material and symbolic violence have radically reshaped the mission and practice of higher education, short-changing a generation of young people. Giroux exposes the corporate forces at play and charts a clear-minded and inspired course of action out of the shadows of market-driven education policy. Championing the youth around the globe who have dared to resist the bartering of their future, he calls upon public intellectualsas well as all people concer ned about the future of democracyto speak out and defend the university as a site of critical learning and democratic promise."

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The dazzling colors and graceful shapes of butterflies make them naturals for tattoo art. Now this array of miniature temporary tattoos allows you to adorn yourself with a host of brilliant butterfly images. Choose from 19 different varieties, among them the longtail skipper, buckeye, giant sulphur, zebra longwing, tortoisehell, pipevine swallowtail, Colorado hairstreak, checkerspot, and 11 more. Each accurately rendered image is perfect for adding a touch of whimsy to arms, legs, faces, and other body parts. In addition, the small size of these charming tattoos makes it possible to cluster them for added effect. Completely safe, the tattoos are easy to apply and remove. Instructions included.

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